Recombinant Human MBL2 protein, His-tagged

• Cat.No.: MBL2-233H
• Product Overview: Recombinant Human MBL2 (Glu21-Ile248) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Mannose-binding lectin (MBL) is also known as mannose-binding protein, mannan-binding protein (MBP), Mannose-binding protein C, Collectin-1 (COLEC1), MBL2, which belongs to the class of collectins in the C-type lectin superfamily. MBL contains one C-type lectin domain and one collagen-like domain. MBL has an oligomeric structure (400-700 kDa), built of subunits that contain three presumably identical peptide chains of about 30 kDa each. MBL is calcium-dependent lectin involved in innate immune defense. MBL binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. MBL binds to late apoptotic cells. MBL may bind DNA.
• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Glu21
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 24.8 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: Glu21-Ile248
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: MBL2
• Official Symbol: MBL2
• Synonyms: MBL2; mannose-binding lectin (protein C) 2, soluble; mannose binding lectin (protein C) 2, soluble (opsonic defect) , MBL; mannose-binding protein C; COLEC1; collectin-1; mannan-binding lectin; mannose-binding lectin 2, soluble (opsonic defect); mannose-binding lectin (protein C) 2, soluble (opsonic defect); MBL; MBP; MBP1; MBL2D; MBP-C; HSMBPC; MGC116832; MGC116833
• Gene ID: 4153
• mRNA Refseq: NM_000242
• Protein Refseq: NP_000233
• MIM: 154545
• UniProt ID: P11226