Recombinant Human MAP2K7 293 Cell Lysate

• Cat.No.: MAP2K7-4508HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for mitogen-activated protein kinase kinase 7 (MAP2K7) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: MAP2K7 mitogen-activated protein kinase kinase 7 [ Homo sapiens ]
• Official Symbol: MAP2K7
• Synonyms: MAP2K7; mitogen-activated protein kinase kinase 7; PRKMK7; dual specificity mitogen-activated protein kinase kinase 7; Jnkk2; MKK7; MEK 7; JNKK 2; MAPKK 7; SAPK kinase 4; MAPK/ERK kinase 7; MAP kinase kinase 7; JNK-activating kinase 2; c-Jun N-terminal kinase kinase 2; stress-activated protein kinase kinase 4; MAPKK7; SAPKK4; SAPKK-4
• Gene ID: 5609
• mRNA Refseq: NM_145185
• Protein Refseq: NP_660186
• MIM: 603014
• UniProt ID: O14733
• Chromosome Location: 19p13.3-p13.2
• Pathway: Activated TLR4 signalling, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; Cellular roles of Anthrax toxin, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; ErbB signaling pathway, organism-specific biosystem; ErbB signaling pathway, organism-specific biosystem; ErbB signaling pathway, conserved biosystem
• Function: ATP binding; JUN kinase kinase activity; MAP kinase kinase activity; magnesium ion binding; mitogen-activated protein kinase kinase kinase binding; nucleotide binding; protein C-terminus binding; protein binding; protein kinase binding; protein phosphatase binding; protein serine/threonine kinase activity; protein tyrosine kinase activity