Recombinant Human lysine demethylase 3A Protein, His tagged

• Cat.No.: KDM3A-067H
• Product Overview: Recombinant human KDM3A protein (Q9Y4C1) (Met 1-Arg 358) with a His tag at the C-terminus was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-358aa
• Description: Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
• Tag: C-His
• Molecular Mass: 41.1 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: KDM3A lysine demethylase 3A [ Homo sapiens (human) ]
• Official Symbol: KDM3A
• Synonyms: KDM3A; lysine (K)-specific demethylase 3A; JMJD1, JMJD1A, jumonji domain containing 1, jumonji domain containing 1A; lysine-specific demethylase 3A; JHMD2A; jumonji C domain containing histone demethylase 2A; KIAA0742; TSGA; testis-specific protein A; jumonji domain containing 1; jumonji domain containing 1A; jumonji domain-containing protein 1A; jumonji C domain-containing histone demethylase 2A; jmjC domain-containing histone demethylation protein 2A; JMJD1; JHDM2A; JMJD1A; DKFZp686A24246; DKFZp686P07111
• Gene ID: 55818
• mRNA Refseq: NM_001146688
• Protein Refseq: NP_001140160
• MIM: 611512
• UniProt ID: Q9Y4C1