| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
133-411 aa |
| Form : |
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
| Bio-activity : |
Mechanism: Lemur tyrosine kinase 3 (LMTK3), a member of the receptor tyrosine kinase (RTK) family, is implicated in breast cancer growth and endocrine resistance.It has been reported that LMTK3 promotes cell invasion, motility, and migration of the MCF-7 breast cancer cell line . To test the bioactivity of LMTK3 ,MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well in serum-free standard DMEM after adding to various concentrations of LMTK3 protein. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly，10 µl of CCK-8 solution was added to each well of the plate，then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 °C. Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown.Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown. |
| Molecular Mass : |
45.9kDa |
| Endotoxin : |
<1.0EU per 1μg (determined by the LAL method). |
| Purity : |
> 95% |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
