Recombinant Human LGMN, His-tagged

• Cat.No.: LGMN-7977H
• Product Overview: Recombinant Human Legumain produced by transfected human cells is a secreted protein with sequence (Ile18Tyr433) of Human Legumain fused with a polyhistidine tag at the Cterminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Description: Legumain is a lysosomal cysteine protease which is a member of the peptidase C13 family. Though it is found in many tissues, it is highly expressed in the kidney, heart, and placenta. Legumain has a strict specificity for hydrolysis of asparaginyl bonds and can also cleave aspartyl bonds slowly, especially under acidic conditions. Overexpression Legumain in tumors is significant for invasion and metastasis. In addition, Legumain may be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system and negative regulation of neuron apoptosis.
• Form: Supplied as a 0.2 μM filtered solution of 20mM TrisHCl, 150mM NaCl, 10% Glycerol, pH 8.0
• AA Sequence: IPIDDPEDGG KHWVVIVAGS NGWYNYRHQA DACHAYQIIH RNGIPDEQIV VMMYDDIAYS EDNPTPGIV INRPNGTDVY QGVPKDYTGE DVTPQNFLAV LRGDAEAVKG IGSGKVLKSG P QDHVFIYF TDHGSTGILV FPNEDLHVKD LNETIHYMYK HKMYRKMVFY IEACESGSMM NH LPDNINV YATTAANPRE SSYACYYDEK RSTYLGDWYS VNWMEDSDVE DLTKETLHKQ YHL VKSHTN TSHVMQYGNK
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Notes: For research use only.
• Storage: Store at less than 20°C. stable for 6 months after receipt.Please minimize freezethaw cycles.

Gene Information

• Gene Name: LGMN legumain [ Homo sapiens ]
• Official Symbol: LGMN
• Synonyms: LGMN; legumain; protease, cysteine, 1 (legumain) , PRSC1; LGMN1; cysteine protease 1; protease, cysteine 1; asparaginyl endopeptidase; protease, cysteine, 1 (legumain); AEP; PRSC1
• Gene ID: 5641
• mRNA Refseq: NM_001008530
• Protein Refseq: NP_001008530
• MIM: 602620
• UniProt ID: Q99538
• Chromosome Location: 14q32.12
• Pathway: Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Metabolism, organism-specific biosystem
• Function: cysteine-type endopeptidase activity; cysteine-type endopeptidase activity; peptidase activity