Recombinant Mouse Lgmn protein(Val18-Tyr435), His-tagged

• Cat.No.: Lgmn-303M
• Product Overview: Recombinant Mouse LGMN (NP_035305.1)(Val 18-Tyr 435) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 18-435 a.a.
• Form: Supplied as sterile 25mM Tris, 0.15M NaCl, 20% Glycerol, pH 7.5.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methyl coumarin(Z-AAN-AMC). The specific activity is > 350 pmoles/min/μg.(Activation description: The enzyme achieves its activity under acidic pH)
• Molecular Mass: The secreted recombinant mouse LGMN consists of 434 amino acids and has a calculated molecular mass of 49.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 75 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Lgmn legumain [ Mus musculus ]
• Official Symbol: Lgmn
• Synonyms: LGMN; legumain; preprolegumain; protease, cysteine 1; protease, cysteine, 1; asparaginyl endopeptidase; AEP; Prsc1; AI746452; AU022324
• Gene ID: 19141
• mRNA Refseq: NM_011175
• Protein Refseq: NP_035305