Recombinant Human KLF6, His-tagged

• Cat.No.: KLF6-5054H
• Product Overview: Recombinant human KLF6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: KLF6, also known as Krueppel-like factor 6, is a nuclear protein that has three zinc fingers. The zinc fingers of this protein are responsible for the specific DNA binding with the guanine-rich core promoter elements. The central region might be involved in activation or posttranslational regulatory pathways, and the acidic N-terminal domain might play an important role in the process of transcriptional activation. It is capable of activating transcription approximately 4-fold either on homologous or heterologous promoters.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 1mM DTT
• Molecular Mass: 34.3 kDa(306aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDVLPMC SIFQELQIVH ETGYFSALPS LEEYWQQTCL ELERYLQSEP CYVSASEIKF DSQEDLWTKI ILAREKKEES ELKISSSPPE DTLISPSFCY NLETNSLNSD VSSESSDSSE ELSPTAKFTS DPIGEVLVSS GKLSSSVTST PPSSPELSRE PSQLWGCVPG ELPSPGKVRS GTSGKPGDKG NGDASPDGRR RVHRCHFNGC RKVYTKSSHL KAHQRTHTGE KPYRCSWEGC EWRFARSDEL TRHFRKHTGA KPFKCSHCDR CFSRSDHLAL HMKRHL
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: KLF6 Kruppel-like factor 6 [ Homo sapiens ]
• Official Symbol: KLF6
• Synonyms: KLF6; Kruppel-like factor 6; BCD1, COPEB, core promoter element binding protein , ST12; Krueppel-like factor 6; CPBP; GBF; GC rich binding factor; PAC1; Zf9; proto-oncogene BCD1; GC-rich binding factor; B-cell-derived protein 1; transcription factor Zf9; protooncogene B-cell derived 1; GC-rich sites-binding factor GBF; Kruppel-like zinc finger protein Zf9; core promoter element binding protein; core promoter element-binding protein; suppressor of tumorigenicity 12 protein; suppression of tumorigenicity 12 (prostate); ZF9; BCD1; CBA1; ST12; COPEB; DKFZp686N0199
• Gene ID: 1316
• mRNA Refseq: NM_001160124
• Protein Refseq: NP_001153596
• MIM: 602053
• UniProt ID: Q99612
• Chromosome Location: 10p15
• Pathway: Adipogenesis, organism-specific biosystem
• Function: DNA binding; double-stranded DNA binding; metal ion binding; sequence-specific DNA binding transcription factor activity; zinc ion binding