Recombinant Human Ketohexokinase

• Cat.No.: KHK-491H
• Product Overview: Recombinant human Ketohexokinase was expressed inE.coliand purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Ketohexokinase is an enzyme that catalyzes the phosphorylation of fructose to produce fructose-1-phosphate, leading to consumption of ATP, formation of AMP. This protein initiates first step in the metabolism of dietary fructose and is an important regulator of hepatic glucose metabolism. It is highly found in liver, renal cortex, and small intestine. Its deficiency causes the benign hereditary metabolic disorder essential fructosuria, leading to fructose being excreted in the urine.
• Sequences of amino acids: MEEKQILCVG LVVLDVISLV DKYPKEDSEI RCLSQRWQRG GNASNSCTIL SLLGAPCAFM GSMAPGHVAD FVLDDLRRYS VDLRYTVFQT TGSVPIATVI INEASGSRTI LYYDRSLPDV SATDFEKVDL TQFKWIHIEG RNASEQVKML QRIDAHNTRQ PPEQKIRVSV EVEKPREELF QLFGYGDVVF VSKDVAKHLG FQSAEEALRG LYGRVRKGAV LVCAWAEEGA DALGPDGKLL HSDAFPPPRV VDTLGAGDTF NASVIFSLSQ GRSVQEALRF GCQVAGKKCG LQGFDGIV
• Form: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol
• Molecular Weight: 32.7 kDa (298aa)
• Purity: > 90% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Fructose and mannose metabolism; Metabolic pathways; Metabolism of carbohydrates

Gene Information

• Gene Name: KHK ketohexokinase (fructokinase) [ Homo sapiens ]
• Synonyms: KHK; ketohexokinase (fructokinase); ketohexokinase; EC 2.7.1.3; ketohexokinase; Hepatic fructokinase
• Gene ID: 3795
• mRNA Refseq: NM_000221
• Protein Refseq: NP_000212
• MIM: 229800
• UniProt ID: P50053
• Chromosome Location: 2p23.3
• Function: ATP binding; ketohexokinase activity; kinase activity; nucleotide binding; protein binding; transferase activity