Recombinant Human ITGAM&ITGB2 protein, His-tagged

• Cat.No.: ITGAM&ITGB2-1688H
• Product Overview: Recombinant Human ITGAM&ITGB2 protein(P11215-1(ITGAM)&P05107-1(ITGB2))(Phe17-Asn1104(ITGAM) & Gln23-Asn700(ITGB2)), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: C-His
• ProteinLength: Phe17-Asn1104(ITGAM) & Gln23-Asn700(ITGB2)
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human ITGAM&ITGB2, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 126.10 kDa(ITGAM)&79.50 kDa(ITGB2). Due to glycosylation, the protein migrates to 140-160 kDa (ITGAM)&90-100 kDa (ITGB2) based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: ITGAM integrin, alpha M (complement component 3 receptor 3 subunit) [ Homo sapiens ]
• Official Symbol: ITGAM
• Synonyms: ITGAM; integrin, alpha M (complement component 3 receptor 3 subunit); CD11B, CR3A, integrin, alpha M (complement component receptor 3, alpha; also known as CD11b (p170), macrophage antigen alpha polypeptide); integrin alpha-M; CD11b; MAC 1; CR-3 alpha chain; antigen CD11b (p170); leukocyte adhesion receptor MO1; CD11 antigen-like family member B; macrophage antigen alpha polypeptide; cell surface glycoprotein MAC-1 subunit alpha; neutrophil adherence receptor alpha-M subunit; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6; MGC117044
• Gene ID: 3684
• mRNA Refseq: NM_000632
• Protein Refseq: NP_000623
• MIM: 120980
• UniProt ID: P11215
• Gene Name 2: ITGB2 integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) [ Homo sapiens ]
• Official Symbol 2: ITGB2
• Synonyms 2: ITGB2; integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); CD18, integrin, beta 2 (antigen CD18 (p95), lymphocyte function associated antigen 1; macrophage antigen 1 (mac 1) beta subunit) , MFI7; integrin beta-2; LFA 1; MAC 1; integrin beta chain, beta 2; complement receptor C3 beta-subunit; complement receptor C3 subunit beta; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; cell surface adhesion glycoprotein LFA-1/CR3/P150,959 beta subunit precursor); LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
• Gene ID 2: 3689
• mRNA Refseq 2: NM_000211
• Protein Refseq 2: NP_000202
• MIM 2: 600065
• UniProt ID 2: P05107