Recombinant HIV-1(group M, subtype CRF07_BC) gp120 protein, His-tagged, Biotinylated

• Cat.No.: gp120-0406H
• Product Overview: Biotinylated Recombinant HIV-1(group M, subtype CRF07_BC) gp120 protein(Ala29-Glu502), fused with His tag, was expressed in HEK293.

Specification

• Species: HIV
• Source: HEK293
• Tag: His
• Protein Length: Ala29-Glu502
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The recombinant HIV-1 gp120 consists of 485 amino acids after removal of the signal peptide and has a predicted molecular mass of 54.8 kDa. It migrates as an approximately 95.49 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.