Recombinant Human ITGA4 protein, His-tagged

• Cat.No.: ITGA4-1541H
• Product Overview: Recombinant Human ITGA4(aa 131 - 275) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 131-275 a.a.
• Description: The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha 4 chain associates with either beta 1 or beta 7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin 41 and 47 is located on the 4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin a4b7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin 47, the gut mucosal homing receptor for peripheral T cells. This recombinant protein fragment includes the ligand and monoclonal antibody binding domains; this binding has been tested and verified in ELISA assays. This recombinant protein is recognized PSI antibodies Integrin 4 (IN1) and Integrin 4 (IN2), but not Integrin 4 (CT)).
• Form: 50mM Tris pH7.5, 150mM NaCl, 5 mM MgCl2
• Molecular Mass: 17 kDa (Calculated)
• Purity: ~95%
• Applications: ELISA, WB. This recombinant protein can be used for WB and ELISA.
• Notes: For research use only.
• Storage: Store in working aliquots at -70 centigrade. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Gene Information

• Gene Name: ITGA4 integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) [ Homo sapiens ]
• Official Symbol: ITGA4
• Synonyms: ITGA4; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); CD49D; integrin alpha-4; CD49d; 269C wild type; integrin alpha 4; integrin alpha-IV; VLA-4 subunit alpha; integrin alpha-4 subunit; CD49 antigen-like family member D; antigen CD49D, alpha-4 subunit of VLA-4 receptor; very late activation protein 4 receptor, alpha 4 subunit; IA4; MGC90518
• Gene ID: 3676
• mRNA Refseq: NM_000885
• Protein Refseq: NP_000876
• MIM: 192975
• UniProt ID: P13612
• Chromosome Location: 2q31-q32
• Pathway: Adaptive Immune System, organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), conserved biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Dilated cardiomyopathy, organism-specific biosystem
• Function: fibronectin binding; protein binding; receptor activity