Recombinant Human interleukin 12 receptor, beta 1 Protein, His tagged

• Cat.No.: IL12RB1-139H
• Product Overview: Recombinant human IL12RB1 protein (P42701) (Cys 24-Glu 540) with a His tag at the C-terminus was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 24-540aa
• Description: Interleukin 12 (IL-12) is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of two subunits designated IL-12 R beta 1 and IL-12 R beta 2. Individually, IL-12 R beta 1 and IL-12 R beta 2 bind IL-12 with low affinity. Co-expression of both subunits confers high-affinity binding and is required for IL-12 activity. Both IL-12 receptor subunits are type I membrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 1 cDNA encodes a 662 amino acid (aa) residue protein with a putative 23 aa residue signal peptide that is cleaved to generate the mature protein with a 522 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 92 aa residue cytoplasmic region. Expression of IL-12 R beta 1 has been detected in activated T cells, NK cells and B cells. The expression of IL-12 R beta 2 is more restricted and appears to be limited to Th2 cells.
• Tag: C-His
• Molecular Mass: 58.5 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: IL12RB1 interleukin 12 receptor, beta 1 [ Homo sapiens (human) ]
• Official Symbol: IL12RB1
• Synonyms: IL12RB1; interleukin 12 receptor, beta 1; IL12RB; interleukin-12 receptor subunit beta-1; CD212; IL-12RB1; IL-12R-beta-1; IL-12R subunit beta-1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; interleukin-12 receptor beta-1 chain; IL-12R-BETA1; MGC34454
• Gene ID: 3594
• mRNA Refseq: NM_005535
• Protein Refseq: NP_005526
• MIM: 601604
• UniProt ID: P42701