Recombinant Human Interleukin 10

• Cat.No.: IL10-217H
• Product Overview: Recombinant Human interleukin 10 DNA sequence encoding the mature human IL-10 protein sequence was inserted into a suitable expression vector and was expressed inSf 21 insect cells.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: Non
• Description: Interleukin-10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is a homodimer protein produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-γ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and the Type 1 T helper cells. IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. It is released by cytotoxic T-cells to inhibit the actions of NK cells during the immune response to viral infection. IL-10 plays a key role in peripheral T cell tolerance to allergens, autoantigens, transplantation antigens and tumor antigens.
• Molecular Weight: The predicted molecular weight of Recombinant Human IL-10 is Mr 18.6 kDa.
• State Of Matter: Lyophilized.
• Formulation: This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
• Purity: > 97% by SDS Page and analyzed by silver stain.
• Endotoxin: < 1.0 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Human IL-10 was determined by a cell proliferation assay using the murine mast cell line, MC/9-2. The expected ED50 for this effect is typically 0.15 - 0.75 ng/mL. The cell number is assessed in a fluorometric assay using the redox senstitive dye, Resazurin.
• Storage And Stability: Working aliquots of this recombinant protein solution are stable for up to twelve months at -20˚C to -70˚C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2˚C - 8˚C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20˚C to -70˚C in a manual defrost freezer. Avoid repeated freeze thaw cycles.

Gene Information

• Gene Name: IL10 interleukin 10 [ Homo sapiens ]
• Synonyms: IL10; interleukin 10; CSIF; TGIF; IL-10; IL10A; MGC126450; MGC126451; Interleukin-10; Cytokine synthesis inhibitory factor; MGC126450; MGC126451
• Gene ID: 3586
• mRNA Refseq: NM_000572
• Protein Refseq: NP_000563
• UniProt ID: P22301
• Chromosome Location: 1q31-q32
• MIM: 124092
• Pathway: Allograft rejection; Asthma; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Systemic lupus erythematosus; T cell receptor signaling pathway
• Function: cytokine activity; growth factor activity; interleukin-10 receptor binding; protein binding