Recombinant Human INS protein

Specification
Gene Information
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Description :	This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants.
Source :	E.coli
Species :	Human
Form :	Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4.
Molecular Mass :	Approximately 3.6 kDa, a single non-glycosylated polypeptide chain containing 35 amino acids.
Protein length :	35
AA Sequence :	RREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKR
Endotoxin :	Less than 0.1 EU/µg of rHuProinsulin C-Peptide Analogue as determined by LAL method.
Purity :	>95% by SDS-PAGE and HPLC analyses.
Storage :	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution :	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Tag :	Non

Gene Name	INS
Official Symbol	INS
Synonyms	INS; insulin; proinsulin; ILPR; IRDN; IDDM2; MODY10;
Gene ID	3630
mRNA Refseq	NM_000207
Protein Refseq	NP_000198
MIM	176730
UniProt ID	P01308