Active Recombinant Human IgG1Fc Protein, Avi-tagged, Biotinylated

• Cat.No.: IgG1Fc-12H
• Product Overview: Biotinylated Human IgG1 Fc is expressed from HEK293 cells. It contains AA Glu 99 - Lys 330 (Accession # P01857-1 ). This protein carries an Avi tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi
• Protein Length: 99-330 a.a.
• Predicted N Terminal: Glu 99
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Recombinant Protein G at 2 ug/mL (100 uL/well) can bind Biotinylated Human IgG1 Fc, Avi Tag with a linear range of 13-512 ug/mL.
• Molecular Mass: The protein has a calculated MW of 28.4 kDa. The protein migrates as 36-40 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Official Symbol: IgG1Fc
• Synonyms: IgG Fc