Active Recombinant Human IgG1Fc protein, His-tagged

• Cat.No.: IgG1Fc-09H
• Product Overview: Recombinant Human IgG1Fc protein(Glu 99 - Lys 330), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 99-330 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 51 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with Trehalose as protectant.
• Bio-activity: Immobilized Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F), His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag with a linear range of 0.4-13 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 28.1 kDa. The protein migrates as 30-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Glu99-Lys330
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.