Active Recombinant Human IgG1Fc protein, Avi-tagged, Biotinylated

• Cat.No.: IgG1Fc-0250H
• Product Overview: Biotinylated Recombinant Human IgG1Fc protein(Glu99-Lys330), fused with Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi
• Protein Length: 99-330 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Recombinant Protein G, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human IgG1 Fc, Avitag with a linear range of 0.013-0.512 μg/mL.
• Molecular Mass: This protein carries an Avi tag (Avitag) at the C-terminusThe protein has a calculated MW of 28.4 kDa. The protein migrates as 32-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Glu99-Lys330
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: IgG1Fc