Recombinant Human IGF1 Protein

• Cat.No.: IGF1-036H
• Product Overview: Recombinant human Insulin-like growth factor 1 (rhIGF-I), Gly49-Ala118 (Accession # P05019) was expressed in human HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: 49-118 a.a.
• Description: The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]
• Molecular Mass: rhIGF-I was fused two amino acid gly-pro at the N-terminal and has a calculated MW of 7.5 kDa. DTT-reduced protein migrates as 8 kDa polypeptide in SDS-PAGE.
• Endotoxin: Less than 1.0 EU endotoxin per µg rhIGF-I by the LAL method.
• Purity: >95% purity as determined by SDS-PAGE of reduced rhIGF-I.
• Notes: Research Use Only. Not for use in diagnostic or therapeutic procedures.
• Storage: No activity loss was observed after storage at 4-8 centigrade for 1 month or -20 centigrade for 1 year in lyophilized state. rhIGF-I should be aliquoted and stored at -8 centigrade centigrade in liquid formulation, for long term storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as a 0.22 µm filtered solution in 50mM Tris, 100 mM NaCl, pH 8.0 and delivered as liquid formulation or lyophilized powder. Normally mannitol or trehalose are added as protectants before lyophilization. Reconstitution instruction or specific concentrations are included in the hardcopy of CoA along with the products.

Gene Information

• Gene Name: IGF1 insulin-like growth factor 1 (somatomedin C) [ Homo sapiens ]
• Official Symbol: IGF1
• Synonyms: IGF1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; IGF1A; MGF; IGF-IA; IGF-IB; somatomedin-C; mechano growth factor; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; IGFI; IGF-I
• Gene ID: 3479
• mRNA Refseq: NM_000618
• Protein Refseq: NP_000609
• MIM: 147440
• UniProt ID: P05019