Recombinant Bovine Igf1 Protein, Gly50-Ala119

• Cat.No.: Igf1-02B
• Product Overview: Recombinant Bovine Igf1 Protein (Gly50-Ala119) without tag was expressed in E. coli.

Specification

• Species: Bovine
• Source: E.coli
• Protein Length: Gly50-Ala119
• Description: Insulin-like growth factor 1 (IGF-1), also called Somatomedin, is a hormone similar in molecular structure to insulin but has a much higher growth-promoting activity. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. It is able to stimulate glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. It may also play a role in synapse maturation.
• Bio-activity: ED50 < 15.0 ng/ml, measured by a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of > 6.7 × 104 units/mg.
• Molecular Mass: ~ 7.7 kDa, on SDS-PAGE under reducing conditions.
• Endotoxin: < 0.2 EU/μg of protein by gel clotting method
• Purity: > 95% as analyzed by SDS-PAGE
• N-terminal Sequence Analysis: Met
• Storage: Upon receiving, this product remains stable for up to 6 months at lower than -70 centigrade. Upon reconstitution, the product should be stable for up to 1 week at 4 centigrade or up to 3 months at -20 centigrade. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O up to 100 μg/ml.

Gene Information

• Gene Name: IGF1 insulin like growth factor 1 [ Bos taurus (cattle) ]
• Official Symbol: IGF1
• Synonyms: IGF1; insulin like growth factor 1; IGF-1; IGF-I; insulin-like growth factor I; class 1 insulin-like growth factor I preproprotein; insulin growth factor-1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor I (somatomedin C); prepro-IGF-I; somatomedin
• Gene ID: 281239
• mRNA Refseq: NM_001077828
• Protein Refseq: NP_001071296
• UniProt ID: P07455