Recombinant Human Hydroxyacyl-Coenzyme A Dehydrogenase, His-tagged

• Cat.No.: HADH-1694H
• Product Overview: Recombinant HADH protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: HADH-1694H
• Description: HADH, which belongs to the family of oxidoreductases, is important for converting certain fats to energy. This protein is an enzyme that catalyzes the chemical reaction; (S)-3-hydroxyacyl-CoA + NAD+ <=>3-oxoacyl-CoA + NADH + H+. It is also involved in a process called fatty acid oxidation, in which several enzymes work in a step-wise fashion to break down (metabolize) fats and convert them to energy.
• Concentration: 1 mg/ml
• Source: Human
• Host: E.coli
• Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol and 0.1M NaCl.
• Purity: > 95% by SDS - PAGE
• Sequence: 13-314 amino acids: MGSSHHHHHH SSGLVPRGSH MSSSSTASAS AKKIIVKHVT VIGGGLMGAG IAQVAAATGH TVVLVDQTED ILAKSKKGIE ESLRKVAKKK FAENPKAGDE FVEKTLSTIA TSTDAASVVH STDLVVEAIV ENLKVKNELF KRLDKFAAEH TIFASNTSSL QITSIANATT RQDRFAGLHF FNPVPVMKLV EVIKTPMTSQ KTFESLVDFS KALG
• Molecular Mass: 35.1 kDa (323aa), confirmed by MALDI-TOF.
• Applications: SDS-PAGE
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: HADH hydroxyacyl-Coenzyme A dehydrogenase [ Homo sapiens ]
• Official Symbol: HADH
• Synonyms: HADH; hydroxyacyl-Coenzyme A dehydrogenase; HAD; HHF4; HADH1; SCHAD; HADHSC; M/SCHAD; MGC8392; HADH; hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; short-chain 3-hydroxyacyl-CoA dehydrogenase; L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain; medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; EC 1.1.1.35; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short chain 3-hydroxyacyl-CoA dehydrogenase; L-3-hydroxyacyl-Coenzyme A dehydrogenase
• Gene ID: 3303
• mRNA Refseq: NM_005327
• Protein Refseq: NP_005318
• MIM: 601609
• UniProt ID: Q16836
• Chromosome Location: 4q22-q26
• Pathway: Butanoate metabolism; Fatty acid elongation in mitochondria; Fatty acid metabolism; Lysine degradation;Metabolic pathways; Tryptophan metabolism; Valine, leucine and isoleucine degradation
• Function: 3-hydroxyacyl-CoA dehydrogenase activity; binding; coenzyme binding; oxidoreductase activity