Recombinant Human HSD17B3 protein, His-tagged

• Cat.No.: HSD17B3-1407H
• Product Overview: Recombinant Human HSD17B3 aa. (Met1~Arg310 fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met1~Arg310
• Description: This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.
• Form: Freeze-dried powder
• Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 8.9.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: HSD17B3 hydroxysteroid 17-beta dehydrogenase 3 [ Homo sapiens (human) ]
• Official Symbol: HSD17B3
• Synonyms: HSD17B3; hydroxysteroid 17-beta dehydrogenase 3; EDH17B3; SDR12C2; testosterone 17-beta-dehydrogenase 3; 17-beta-HSD 3; 17-beta-HSD3; 17-beta-hydroxysteroid dehydrogenase type 3; hydroxysteroid dehydrogenase 3; short chain dehydrogenase/reductase family 12C member 2; testicular 17-beta-hydroxysteroid dehydrogenase
• Gene ID: 3293
• mRNA Refseq: NM_000197.1
• Protein Refseq: NP_000188.1
• UniProt ID: P37058