Recombinant Human HSD17B3 293 Cell Lysate

• Cat.No.: HSD17B3-5374HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for hydroxysteroid (17-beta) dehydrogenase 3 (HSD17B3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: HSD17B3 hydroxysteroid (17-beta) dehydrogenase 3 [ Homo sapiens ]
• Official Symbol: HSD17B3
• Synonyms: HSD17B3; hydroxysteroid (17-beta) dehydrogenase 3; testosterone 17-beta-dehydrogenase 3; SDR12C2; short chain dehydrogenase/reductase family 12C; member 2; 17-beta-HSD3; 17-beta-HSD 3; 17-beta-hydroxysteroid dehydrogenase type 3; testicular 17-beta-hydroxysteroid dehydrogenase; short chain dehydrogenase/reductase family 12C, member 2; EDH17B3
• Gene ID: 3293
• mRNA Refseq: NM_000197
• Protein Refseq: NP_000188
• MIM: 605573
• UniProt ID: P37058
• Chromosome Location: 9q22
• Pathway: Androgen biosynthesis, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Metabolism of steroid hormones and vitamins A and D, organism-specific biosystem; Steroid Biosynthesis, organism-specific biosystem; Steroid hormone biosynthesis, organism-specific biosystem
• Function: estradiol 17-beta-dehydrogenase activity; nucleotide binding; oxidoreductase activity; testosterone 17-beta-dehydrogenase (NAD+) activity; testosterone 17-beta-dehydrogenase (NADP+) activity