Recombinant Human HMGB1 Protein, His-tagged

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Product Overview :	Recombinant Human HMGB1 Protein (Gly2-Arg97) with a N-His tag was expressed in E. coli.
Description :	This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.
Source :	E. coli
Species :	Human
Tag :	His
Form :	Freeze-dried powder
Molecular Mass :	Predicted Molecular Mass: 12.8 kDa Accurate Molecular Mass: 16 kDa
Protein length :	Gly2-Arg97
Endotoxin :	<1.0 EU per 1µg (determined by the LAL method).
Purity :	> 95%
Applications :	Positive Control; Immunogen; SDS-PAGE; WB.
Stability :	The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage :	Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Storage Buffer :	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Reconstitution :	Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Name	HMGB1 high mobility group box 1 [ Homo sapiens (human) ]
Official Symbol	HMGB1
Synonyms	HMGB1; high mobility group box 1; high mobility group (nonhistone chromosomal) protein 1, high mobility group box 1, HMG1; high mobility group protein B1; Amphoterin; DKFZp686A04236; high mobility group protein 1; HMG3; SBP 1; Sulfoglucuronyl carbohydrate binding protein; HMG-1; high-mobility group box 1; high-mobility group (nonhistone chromosomal) protein 1; HMG1; SBP-1;
Gene ID	3146
mRNA Refseq	NM_002128
Protein Refseq	NP_002119
MIM	163905
UniProt ID	P09429