Recombinant Human HLA 293 Cell Lysate

• Cat.No.: HLA-DRB5-5497HCL

Specification

• Description: Antigen standard for major histocompatibility complex, class II, DR beta 5 (HLA-DRB5) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: HLA-DRB5 major histocompatibility complex, class II, DR beta 5 [ Homo sapiens ]
• Official Symbol: HLA-DRB5
• Synonyms: HLA-DRB; major histocompatibility complex, class II, DR beta 5; DR9; dw2; DR-9; DR beta-5; DR2-beta-2; MHC HLA-DR-beta chain; MHC class II antigen DRB5; leukocyte antigen class II; MHC class II HLA beta chain; MHC class II antigen DRB1*9; human leucocyte antigen; DRB5; MHC HLA-DR-beta cell surface glycoprotein; HLA class II histocompatibility antigen, DR beta 5 chain; HLA class II; histocompatibility antigen, DR-5 beta chain; HLA class II histocompatibility antigen, DRB1-9 beta chain
• Gene ID: 3127
• mRNA Refseq: NM_002125
• Protein Refseq: NP_002116
• MIM: 604776
• UniProt ID: Q30154
• Chromosome Location: 6p21.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Costimulation by the CD28 family, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Downstream TCR signaling, organism-specific biosystem; Generation of second messenger molecules, organism-specific biosystem; Immune System, organism-specific biosystem; Interferon Signaling, organism-specific biosystem
• Function: peptide antigen binding