Recombinant Human Histamine Receptor H 2

• Cat.No.: HRH2-1047H

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: Non
• Description: The histamine H2-receptor (H₂R) belongs to the superfamily of seven transmembrane domain (7TM), G-protein-coupled receptors (GPCRs). The endogenous agonist for the H₂R is histamine which is both neurotransmitter and autacoid.The H₂R couples to Gs-proteins to mediate adenylate cyclase (AC) activation. In some systems, the H₂R also couples to Gq-proteins and phospholipase C. Among the many responses mediated by the H₂R are gastric acid secretion, smooth muscle relaxation, positive ionotropic and chronotropic effects on heart muscle, and inhibition of leukocyte function. The guinea pig H₂R (gp H₂R) was prepared fromSf9 cellsinfected with gp H₂R -encoding baculovirus. In this system, gp H₂R couples to the Gs-like G-proteins of the insect cells to mediate AC activation. gp H₂R contains a N-terminal FLAG-tag and a Cterminal hexahistidine (His6)-tag for immunological detection, to allow purification, and to provide additional protection against proteolysis.
• Form: Membrane suspension. Supplied in 75 mM, Tris-HCl pH 7.4, 12.5 mM MgCl₂and 1 mM EDTA.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months. Store at -80°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: HRH2 histamine receptor H 1 [ Cavia porcellus ]
• Synonyms: HRH2; histamine receptor H 2; Histamine H2 receptor; Gastric receptor I; gastric receptor 1; DBP1; DDX15; DHX15; H2R
• Gene ID: 3274
• mRNA Refseq: NM_001131055
• Protein Refseq: NP_001124527
• MIM: 142703
• UniProt ID: P25021
• Chromosome Location: 5q35
• Pathway: Calcium signaling pathway; Neuroactive ligand-receptor interaction; Signaling by GPCR
• Function: G-protein coupled receptor activity; histamine receptor activity; receptor activity