Recombinant Human HIST2H3A protein, His-tagged

• Cat.No.: HIST2H3A-1559H
• Product Overview: Recombinant Human HIST2H3A aa. (Met1~Ala136 (Accession # Q71DI3)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.9kDa
• Protein length: Met1~Ala136
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 11.1

Gene Information

• Gene Name: HIST2H3A histone cluster 2 H3 family member a [ Homo sapiens (human) ]
• Official Symbol: HIST2H3A
• Synonyms: HIST2H3A; histone cluster 2 H3 family member a; H3/n; H3/o; histone H3.2; histone 2, H3a; histone H3/o; histone cluster 2, H3a
• Gene ID: 333932
• mRNA Refseq: NM_001005464.2
• Protein Refseq: NP_001005464.1
• UniProt ID: Q71DI3