Recombinant Human HIST2H2AC Protein, His-tagged

• Cat.No.: HIST2H2AC-2241H
• Product Overview: Recombinant Human HIST2H2AC Protein (Met1-Lys129) with a N-His tag was expressed in E. coli.

Specification

• Description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 17.7 kDa
  Accurate Molecular Mass: 18 kDa
• Protein length: Met1-Lys129
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: HIST2H2AC histone cluster 2, H2ac [ Homo sapiens (human) ]
• Official Symbol: HIST2H2AC
• Synonyms: H2AC20; H2A clustered histone 20; H2A; H2A/q; H2AFQ; H2A-GL101; HIST2H2AC; histone H2A type 2-C; H2A histone family, member Q; histone 2, H2ac; histone H2A-GL101; histone H2A/q; histone IIa; histone cluster 2 H2A family member c; histone cluster 2, H2ac
• Gene ID: 8338
• mRNA Refseq: NM_003517
• Protein Refseq: NP_003508
• MIM: 602797
• UniProt ID: Q16777