Recombinant Human HIST1H2AB protein, His & GST-tagged

• Cat.No.: HIST1H2AB-1516H
• Product Overview: Recombinant Human HIST1H2AB aa. (Ser2~Lys130 (Accession # P04908)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 41.1kDa
• Protein length: Ser2~Lys130
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 9.5

Gene Information

• Gene Name: HIST1H2AB histone cluster 1 H2A family member b [ Homo sapiens (human) ]
• Official Symbol: HIST1H2AB
• Synonyms: HIST1H2AB; histone cluster 1 H2A family member b; H2A/m; H2AFM; histone H2A type 1-B/E; H2A histone family, member M; histone 1, H2ab; histone H2A/m; histone cluster 1, H2ab
• Gene ID: 8335
• mRNA Refseq: NM_003513.2
• Protein Refseq: NP_003504.2
• UniProt ID: P04908