Recombinant Human High-mobility Group Box 1, His-tagged

• Cat.No.: HMGB1-1984H
• Product Overview: Recombinant human HMGB1(a.a. 223) fused to His-Tag is produced inSf9insect cells, 25 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: HMGB1 is an abundant chromatin-binding protein found in eukaryotic cell nucleus and acts in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and is involved in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine with activities that resemble those of tumor necrosis factor (TNF). HMGB1 is elevated significantly in chronic kidney disease patients and correlates with glomerular filtration rate as well as with markers of inflammation and malnutrition. HMGB1 is involved in Gram-negative sepsis by catalyzing movement of LPS monomers from LPS aggregates to CD14 to initiate a TLR4-mediated proinflammatory response. HMGB1 plays an important role in the relationship between necrosis and malignancy in glioma tumours.
• Amino Acid Sequence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH.
• Physical Appearance: Sterile Filtered colorless solution.
• Purity: Greater than 90% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The HMG1 solution (1mg/ml) contains 20mM Tris pH-8, 1mM EDTA, 0.5mM DTT and 10% glycerol.
• Storage: HMG1 although stable 14°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Pathways: Base excision repair

Gene Information

• Gene Name: HMGB1 high-mobility group box 1 [ Homo sapiens ]
• Synonyms: HMGB1; high-mobility group box 1; HMG1; HMG3; SBP-1; DKFZp686A04236; Sulfoglucuronyl carbohydrate binding protein; Amphoterin; high mobility group protein 1; High mobility group protein B1; High mobility group protein 1; HMG-1; DKFZp686A04236; OTTHUMP00000190860; OTTHUMP00000018199; high-mobility group (nonhistone chromosomal) protein 1
• Gene ID: 3146
• mRNA Refseq: NM_002128
• Protein Refseq: NP_002119
• MIM: 163905
• UniProt ID: P09429
• Chromosome Location: 13q12
• Function: DNA bending activity; transcription factor binding