Recombinant Human heparanase, His-tagged

• Cat.No.: HPSE-467H
• Product Overview: HPSE, 36-543aa Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 36-543 a.a.
• Description: Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. HPSE is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Recombinant human HPSE protein, fused to His-tag at N-terminus, was expressed in E.coli .
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea
• Molecular Mass: 60.0 kDa (531aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSQDVVDLD FFTQEPLHLV SPSFLSVTID ANLATDPRFL ILLGSPKLRT LARGLSPAYL RFGGTKTDFL IFDPKKESTF EERSYWQSQV NQDICKYGSI PPDVEEKLRL EWPYQEQLLL REHYQKKFKN STYSRSSVDV LYTFANCSGL DLIFGLNALL RTADLQWNSS NAQLLLDYCS SKGYNISWEL GNEPNSFLKK ADIFINGSQL GEDFIQLHKL LRKSTFKNAK LYGPDVGQPR RKTAKMLKSF LKAGGEVIDS VTWHHYYLNG RTATKEDFLN PDVLDIFISS VQKVFQVVES TRPGKKVWLG ETSSAYGGGA PLLSDTFAAG FMWLDKLGLS ARMGIEVVMR QVFFGAGNYH LVDENFDPLP DYWLSLLFKK LVGTKVLMAS VQGSKRRKLR VYLHCTNTDN PRYKEGDLTL YAINLHNVTK YLRLPYPFSN KQVDKYLLRP LGPHGLLSKS VQLNGLTLKM VDDQTLPPLM EKPLRPGSSL GLPAFSYSFF VIRNAKVAAC I
• Purity: >85 % by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: HPSE heparanase [ Homo sapiens ]
• Official Symbol: HPSE
• Synonyms: HPSE; heparanase; HPA; HPSE1; HSE1; heparanase-1; endo-glucoronidase; HPA1; HPR1
• Gene ID: 10855
• mRNA Refseq: NM_006665
• Protein Refseq: NP_006656
• MIM: 604724
• UniProt ID: Q9Y251
• Chromosome Location: 4q21.3
• Pathway: Glycosaminoglycan degradation, organism-specific biosystem; Glycosaminoglycan degradation, conserved biosystem; Heparan sulfate degradation, organism-specific biosystem; Heparan sulfate degradation, conserved biosystem; Metabolic pathways, organism-specific biosystem; Syndecan-1-mediated signaling events, organism-specific biosystem
• Function: beta-glucuronidase activity; cation binding; hydrolase activity, acting on glycosyl bonds; protein binding; protein dimerization activity; syndecan binding