Recombinant Human Heme Oxygenase 1, His-tagged

• Cat.No.: HMOX1-1703H
• Product Overview: Recombinant human Heme oxygenase 1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: HMOX1-1703H
• Description: Heme oxygenase 1 belongs to the heme oxygenase family and is an essential enzyme in heme catabolism. It cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Also this protein is known to play an important role in the regulation of cardiovascular function and its adaptive response to a variety of stressors.
• Concentration: 1 mg/ml
• Source: Human
• Host: E.coli
• Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 50mM NaCl, 0.1mM PMSF, and 10% glycerol.
• Purity: > 95% by SDS - PAGE
• Sequence: 1-266 amino acids: MERPQPHSMP QDLSEALKEA TKEVHTQAEN AEFMRNFQKG QVTRDGFKLV MASLYHIYVA LEEEIERNKE SPVFAPVYFP EELHRKAALE QDLAFWYGPR WQEVIPYTPA MQRYVKRLHE VGRTEPELLV AHAYTRYLGD LSGGQVLKKI AQKALDLPSS GEGLAFFTFP NIASATKFKQ LYRSRMNSLE MTPAVRQRVI EEAKTAFLLN IQLF
• Molecular Mass: 31.4 kDa (274 aa), confirmed by MALDI-TOF.
• Applications: SDS-PAGE
• Endotoxin Levels: < 1.0 EU per ug of protein (determined by LAL method)
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: HMOX1 heme oxygenase (decycling) 1 [ Homo sapiens ]
• Official Symbol: HMOX1
• Synonyms: HMOX1; EC 1.14.99.3; HO; HO-1; HO1; HSP32; OTTHUMP00000028925; bK286B10; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; Heme oxygenase 1
• Gene ID: 3162
• mRNA Refseq: NM_002133
• Protein Refseq: NP_002124
• MIM: 141250
• UniProt ID: P09601
• Chromosome Location: 22q12; 22q13.1
• Pathway: Porphyrin and chlorophyll metabolism
• Function: enzyme binding; heme binding; heme oxygenase (decyclizing) activity; metal ion binding; oxidoreductase activity; phospholipase D activity; signal transducer activity

Reviews

10/03/2021

A reliable source for top-notch proteins. Fast shipping is a big plus.

04/14/2020

Exceptional service! This product has become a staple in our lab.

09/30/2018

Impressed with the product's quality. Shipping is always on time.

Q&As

Can you discuss the role of HMOX1 in modulating the immune response in the context of infectious diseases, and how its induction may impact host-pathogen interactions?

HMOX1 modulates the immune response during infectious diseases, influencing host-pathogen interactions. Its induction can contribute to an anti-inflammatory environment and impact the outcome of infections.

How does HMOX1 contribute to the regulation of iron homeostasis, and what are the molecular mechanisms involved in its interaction with iron-responsive elements?

HMOX1 regulates iron homeostasis by releasing free iron during heme degradation. Its interaction with iron-responsive elements influences cellular iron levels and responses to iron availability.

What is the relationship between HMOX1 and oxidative stress-induced autophagy, and how does this connection influence cellular responses to stressors?

HMOX1 is linked to oxidative stress-induced autophagy, contributing to cellular adaptation and survival. This connection influences the cellular response to stressors by promoting self-cleaning mechanisms.

Can you discuss the role of HMOX1 in vascular health and its impact on endothelial function, particularly in the context of cardiovascular diseases?

HMOX1 plays a role in vascular health by protecting endothelial cells. Its anti-inflammatory and vasodilatory effects contribute to cardiovascular protection and may have implications for disease prevention.

How does HMOX1 participate in the modulation of the immune response, and what are the mechanisms by which it influences inflammatory pathways?

HMOX1 modulates the immune response by suppressing inflammation. It inhibits pro-inflammatory pathways by influencing transcription factors and inhibiting the production of inflammatory mediators.