Recombinant Human ALOX5 protein, His-tagged

• Cat.No.: ALOX5-5744H
• Product Overview: Recombinant Human ALOX5 protein(1-349 aa), fused with N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His
• Protein length: 1-349 aa
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization.
• AASequence: MPSYTVTVATGSQWFAGTDDYIYLS LVGSAGCSEKHLLDKPFYNDFERGA VDSYDVTVDEELGEIQLVRIEKRKY WLNDDWYLKYITLKTPHGDYIEFPC YRWITGDVEVVLRDGRAKLARDDQI HILKQHRRKELETRQKQYRWMEWNP GFPLSIDAKCHKDLPRDIQFDSEKG VDFVLNYSKAMENLFINRFMHMFQS SWNDFADFEKIFVKISNTISERVMN HWQEDLMFGYQFLNGCNPVLIRRCT ELPEKLPVTTEMVECSLERQLSLEQ EVQQGNIFIVDFELLDGIDANKTDP CTLQFLAAPICLLYKNLANKIVPIA IQLNQIPGDENPIFLPSDAKYDWL
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks). Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 μg/μl in 200 μl sterile water for short-term storage. After reconstitution with sterile water, if glycerol has no effect on subsequent experiments, it is recommended to add an equal volume of glycerol for long-term storage.

Gene Information

• Gene Name: ALOX5 arachidonate 5-lipoxygenase [ Homo sapiens ]
• Official Symbol: ALOX5
• Synonyms: ALOX5; arachidonate 5-lipoxygenase; 5 LOX; leukotriene A4 synthase; arachidonic acid 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; 5-LO; 5LPG; LOG5; 5-LOX; MGC163204
• Gene ID: 240
• mRNA Refseq: NM_000698
• Protein Refseq: NP_000689
• MIM: 152390
• UniProt ID: P09917

Reviews

05/09/2022

After ALOX5 application, the background of the Western blot was very clean with no nonspecific signal.

08/10/2020

ALOX5 significantly improved the experimental accuracy and made the experimental results more reliable.

05/07/2020

I appreciate the stability of ALOX5, which can be kept at room temperature for several days without spoilage.

Q&As

On which chromosome is the ALOX5 coding gene?

The ALOX5 coding gene is located on human chromosome 10.

What are ALOX5 inhibitors? What is their mechanism of action?

Currently known ALOX5 inhibitors include trypanosomamine, Ritopane, MK-886, etc., whose mechanism of action is to interfere with the expression or function of ALOX5 in different ways, thereby playing an anti-inflammatory or anti-tumor role.

What biological processes is ALOX5 involved in?

ALOX5 is involved in a variety of biological processes, including apoptosis, cell proliferation, inflammatory response, and cancer metastasis.

What factors can regulate ALOX5 gene expression?

The gene expression can be regulated by a variety of factors such as prostaglandins, cytokines and interferons.

Which foods inhibit ALOX5 activity?

Some foods rich in polyphenols, such as cucumbers, tomatoes, grapes, green tea, etc., are thought to inhibit ALOX5 activity.

What cell types have high levels of ALOX5 gene expression?

In general, the ALOX5 gene is highly expressed in eosinophils and monocytes.

What is the function of the ALOX5 promoter region?

The ALOX5 promoter region is the starting point of gene transcription and is responsible for mediating the transcription regulation of the ALOX5 gene.

How to inhibit the effect of ALOX5 in tumor treatment with drugs?

Drugs that block the ALOX5 signaling pathway, such as Zileuton, MK886, and A861, have been shown to inhibit tumor cell proliferation and invasion. Currently, there are also many researchers who are exploring ALOX5 in tumors.

What diseases can ALOX5 gene mutation cause?

ALOX5 gene mutation may cause asthma, allergic rhinitis, eczema and other allergic diseases.

What is 5-HETE? How is it related to ALOX5?

5-HETE is one of the representative products of ALOX5 catalyzed synthesis, and is an activator of platelets and neutrophils.

What is the specific mechanism by which ALOX5 mediates the occurrence of human cancer?

There is no definite answer to this question. Current studies believe that ALOX5 can affect the occurrence, development and metastasis of tumors through multiple ways, including regulating the proliferation, apoptosis, invasion and metastasis of tumor cells.

In what areas can ALOX5 knockout mouse models be used for research?

ALOX5 knockout mouse models can be used to study a variety of biological processes and diseases, such as immune regulation, inflammation, asthma, eczema, autoimmunity, etc.

How to detect the expression of ALOX5 gene?

At present, the commonly used methods include real-time fluorescent quantitative PCR, Northern blot, Western blot, etc.