Recombinant Human HECTD3 protein, His-tagged

• Cat.No.: HECTD3-2442H
• Product Overview: Recombinant Human HECTD3 protein(3-213 aa), fused to His tag, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 3-213 aa
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• AA Sequence: VMEGMDKETFEFKFGKELTFTTVLSDQQVVELIPGGAGIVVGYGDRSRFIQLVQKARLEESKEQVAAMQAGLLKVVPQAVLDLLTWQELEKKVCGDPEVTVDALRKLTRFEDFEPSDSRVQYFWEALNNFTNEDRSRFLRFVTGRSRLPARIYIYPDKLGYETTDALPESSTCSSTLFLPHYASAKVCEEKLRYAAYNCVAIDTDMSPWEE
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: HECTD3 HECT domain containing E3 ubiquitin protein ligase 3 [ Homo sapiens ]
• Official Symbol: HECTD3
• Synonyms: HECTD3; HECT domain containing E3 ubiquitin protein ligase 3; HECT domain containing 3; E3 ubiquitin-protein ligase HECTD3; FLJ21156; HECT domain-containing protein 3; probable E3 ubiquitin-protein ligase HECTD3; RP11-69J16.1; FLJ31983; MGC161630
• Gene ID: 79654
• mRNA Refseq: NM_024602
• Protein Refseq: NP_078878
• UniProt ID: Q5T447