Recombinant Human HBV Core Protein

• Cat.No.: HBcAg-15H
• Product Overview: Recombinant Human HBV core protein produced inE. colihas a molecular mass of 16823 Da.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA (pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.
• Purity: ≥95% as determined by SDS-PAGE.
• Concentration: 0.2 mg / ml by BCA
• Component: HBV core protein, NaHCO₃10μl, pH 7.4
• Specific Activity: Immunoreactive with sera HBV-infected individuals.
• Endotoxin: less than 10EU/mg as determined by LAL method
• Applications: HBV Core antigen is stable in ELISA and Western blots, excellent antigen for detection of HBV with minimal specificity problems.
• Storage buffter: Liquid. In NaHCO₃buffer
• Storage: HBV Core protein is shipped at ambient temperature. Upon arrival, Store at -20°C . Five years frozen. One month in solution at room temperature.