Recombinant Human HAO1 293 Cell Lysate

• Cat.No.: HAO1-5638HCL

Specification

• Description: Antigen standard for hydroxyacid oxidase (glycolate oxidase) 1 (HAO1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: HAO1 hydroxyacid oxidase (glycolate oxidase) 1 [ Homo sapiens ]
• Official Symbol: HAO1
• Synonyms: HAO1; hydroxyacid oxidase (glycolate oxidase) 1; GOX1; hydroxyacid oxidase 1; GOX; glycolate oxidase; (S)-2-hydroxy-acid oxidase; HAOX1; MGC142225; MGC142227
• Gene ID: 54363
• mRNA Refseq: NM_017545
• Protein Refseq: NP_060015
• MIM: 605023
• UniProt ID: Q9UJM8
• Chromosome Location: 20p12
• Pathway: Glyoxylate and dicarboxylate metabolism, organism-specific biosystem; Glyoxylate and dicarboxylate metabolism, conserved biosystem; Glyoxylate metabolism, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Peroxisome, organism-specific biosystem
• Function: (S)-2-hydroxy-acid oxidase activity; FMN binding; glycolate oxidase activity; glycolate oxidase activity; glyoxylate oxidase activity; long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity; medium-chain-(S)-2-hydroxy-acid oxidase activity; oxidoreductase activity; very-long-chain-(S)-2-hydroxy-acid oxidase activity

Reviews

05/09/2022

Worked well in experiments.

11/28/2020

Great for academic research.

01/28/2020

Very satisfied, will buy again.

Q&As

How is HAO1 implicated in metabolic disorders?

Dysregulation of HAO1 is linked to metabolic disorders and fatty liver disease.

Are there existing or potential therapeutic strategies targeting HAO1?

Therapeutic approaches may include modulation of HAO1 activity or expression.

How does HAO1 contribute to fatty acid metabolism?

It oxidizes specific fatty acids, contributing to their breakdown.

What are the key biological functions of HAO1?

HAO1 is involved in peroxisomal fatty acid oxidation, crucial for lipid metabolism.

What are the potential consequences of HAO1 overexpression?

HAO1 overexpression may lead to oxidative stress and liver dysfunction.

What role does HAO1 play in liver function?

HAO1 supports liver function by participating in lipid catabolism.

How is HAO1 activity regulated in cells?

Cellular regulation of HAO1 involves transcriptional and post-translational mechanisms.