Recombinant Human HAO1 protein, His-tagged

• Cat.No.: HAO1-378H
• Product Overview: Recombinant Human HAO1 protein (Leu2-Ile370), fused to His tag at N-terminus, was expressed in human 293 cells (HEK293).

Specification

• Description: Hydroxyacid oxidase 1 (HAO-1), a member of the FMN-dependent alpha-hydroxy acid dehydrogenase family, is also known as glycolate oxidase (GOX), which contains 1 FMN hydroxy acid dehydrogenase domain. HAO-1 Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 41.7 kDa. The protein migrates as 41.7 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: 369
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: HAO1
• Official Symbol: HAO1
• Synonyms: HAO1; hydroxyacid oxidase (glycolate oxidase) 1; GOX1; hydroxyacid oxidase 1; GOX; glycolate oxidase; (S)-2-hydroxy-acid oxidase; HAOX1; MGC142225; MGC142227
• Gene ID: 54363
• mRNA Refseq: NM_017545
• Protein Refseq: NP_060015
• MIM: 605023
• UniProt ID: Q9UJM8

Reviews

05/09/2022

Worked well in experiments.

11/28/2020

Great for academic research.

01/28/2020

Very satisfied, will buy again.

Q&As

How is HAO1 implicated in metabolic disorders?

Dysregulation of HAO1 is linked to metabolic disorders and fatty liver disease.

Are there existing or potential therapeutic strategies targeting HAO1?

Therapeutic approaches may include modulation of HAO1 activity or expression.

How does HAO1 contribute to fatty acid metabolism?

It oxidizes specific fatty acids, contributing to their breakdown.

What are the key biological functions of HAO1?

HAO1 is involved in peroxisomal fatty acid oxidation, crucial for lipid metabolism.

What are the potential consequences of HAO1 overexpression?

HAO1 overexpression may lead to oxidative stress and liver dysfunction.

What role does HAO1 play in liver function?

HAO1 supports liver function by participating in lipid catabolism.

How is HAO1 activity regulated in cells?

Cellular regulation of HAO1 involves transcriptional and post-translational mechanisms.