Recombinant Human H2AFX, GST-tagged

• Cat.No.: H2AFX-13646H
• Product Overview: Recombinant Human H2AFX protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 1-143a.a.
• Description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: H2AFX H2A histone family, member X [ Homo sapiens ]
• Official Symbol: H2AFX
• Synonyms: H2AFX; H2A histone family, member X; H2AX; histone H2A.x; H2AX histone; H2A.X; H2A/X
• Gene ID: 3014
• mRNA Refseq: NM_002105
• Protein Refseq: NP_002096
• MIM: 601772
• UniProt ID: P16104
• Chromosome Location: 11q23.3
• Pathway: ATM mediated phosphorylation of repair proteins, organism-specific biosystem; ATM mediated response to DNA double-strand break, organism-specific biosystem; Amyloids, organism-specific biosystem; Assembly of the RAD50-MRE11-NBS1 complex at DNA double-strand breaks, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; DNA Repair, organism-specific biosystem
• Function: DNA binding; enzyme binding; histone binding; protein binding