Recombinant Human GPX4 Protein, 170 residues

• Cat.No.: GPX4-21H
• Product Overview: Recombinant Human Glutathione Peroxidase 4 (Non-tagged; 170 residues)

Specification

• Description: The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Transcript variants resulting from alternative splicing or use of alternate promoters have been described to encode isoforms with different subcellular localization.
• Species: Human
• Protein length: 170 residues
• Purity: ≥ 95% as judged by gel analysis
• Unit definition: 1 U of enzyme consumes 1 μmol GSH per min in a Glutathione Reductase-coupled GPx assay containing 1 mM GSH, 0.5 mM Cumene Hydroperoxide, 15 nM Human Glutathione Reductase and 0.3 mM NADPH in TE buffer (50 mM Tris-HCl, 2 mM EDTA, pH 7.5)
• Applications: Glutathione Reductase-coupled GPx assay; Inhibitor screening; etc.
• Notes: Centrifuge the tube briefly before opening the lid.
• Concentration: 1 mg/mL
• Storage Buffer: 25 mM Tris-HCl (pH 8.0) with 250 mM KCl, 2 mM 2-Mercaptoethanol and 10% glycerol.
• Shipping: Shipped in cold pack by courier service. Store at -20 centigrade upon delivery.
• References: 1. Cheng Q, Arnér ES. (2017) Selenocysteine Insertion at a Predefined UAG Codon in a Release Factor 1 (RF1)-depleted Escherichia coli Host Strain Bypasses Species Barriers in Recombinant Selenoprotein Translation. J Biol Chem. 292:5476-5487.

Gene Information

• Gene Name: GPX4 glutathione peroxidase 4 [ Homo sapiens (human) ]
• Official Symbol: GPX4
• Synonyms: GPX4; glutathione peroxidase 4; glutathione peroxidase 4 (phospholipid hydroperoxidase); phospholipid hydroperoxide glutathione peroxidase, mitochondrial; MCSP; PHGPx; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase; GPx-4; snGPx; GSHPx-4; snPHGPx
• Gene ID: 2879
• mRNA Refseq: NM_001039847
• Protein Refseq: NP_001034936
• MIM: 138322
• UniProt ID: P36969