Recombinant Human GPX4 Protein

Cat.No. : GPX4-23H
Product Overview : Recombinant Human Glutathione Peroxidase 4 (Non-tagged; 170 residues)
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Species : Human
Tag : Non
Description : The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Transcript variants resulting from alternative splicing or use of alternate promoters have been described to encode isoforms with different subcellular localization.
Purity : ≥ 95% as judged by gel analysis
Unit Definition : 1 U of enzyme consumes 1 μmol GSH per min in a Glutathione Reductase-coupled GPx assay containing 1 mM GSH, 0.5 mM Cumene Hydroperoxide, 15 nM Human Glutathione Reductase and 0.3 mM NADPH in TE buffer (50 mM Tris-HCl, 2 mM EDTA, pH 7.5)
Applications : Glutathione Reductase-coupled GPx assay; Inhibitor screening; etc.
Notes : Centrifuge the tube briefly before opening the lid
Storage : Store at -20 centigrade upon delivery.
Concentration : 1 mg/mL
Storage Buffer : 25 mM Tris-HCl (pH 8.0) with 250 mM KCl, 2 mM 2-Mercaptoethanol and 10% glycerol.
Shipping : Shipped in cold pack by courier service.
Gene Name GPX4 glutathione peroxidase 4 [ Homo sapiens (human) ]
Official Symbol GPX4
Synonyms GPX4; glutathione peroxidase 4; MCSP; SMDS; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGP; phospholipid hydroperoxide glutathione peroxidase; epididymis secretory sperm binding protein; phospholipid hydroperoxidase; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; selenoprotein GPX4; sperm nucleus glutathione peroxidase; EC 1.11.1.12
Gene ID 2879
mRNA Refseq NM_002085
Protein Refseq NP_002076
MIM 138322
UniProt ID P36969

Not For Human Consumption!

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