Recombinant Human GPC2 protein, His-tagged

• Cat.No.: GPC2-3829H
• Product Overview: Recombinant Human GPC2 protein(Val338~Thr562), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N-His
• Protein length: Val338~Thr562
• Form: PBS, pH7.4, containing 5% Trehalose.
• Bio-activity: Glypican 2 (GPC2), a member of glypican (GPC) family genes, produces proteoglycan with a glycosylphosphatidylinositol anchor. Level of the various glypicans changes in a stage and tissue specific manner. Syndecan-2 is expressed in cells of mesenchymal origin, neuronal and epithelial cells, and is the predominant syndecan expressed during embryonic development. Human Syndecan-2 is synthesized as a 201 amino acid (aa) core protein with an 18 aa signal sequence, a 126 aa extracellular domain (ECD), a 25 aa transmembrane region and a 32 aa cytoplasmic tail. The human ECD of Syndecan-2 contains three closely-spaced consensus Ser-Gly sequences for the attachment of HS side chains. It shares 76%, 73%, 87%, 78% and 63% aa identity with the ECD of mouse, rat, bovine, canine and chicken Syndecan-2, respectively. SDC1 has been identified as an interactor of GPC2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GPC2 and recombinant bovine SDC1. Briefly, biotin-linked GPC2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to SDC1-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 μl stop solution to the wells and read at 450 nm immediately. The EC50 is 195.6 ng/mL.
• Molecular Mass: 25.6kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method)
• Purity: > 95%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: GPC2 glypican 2 (cerebroglycan) [ Homo sapiens ]
• Official Symbol: GPC2
• Synonyms: GPC2; glypican 2 (cerebroglycan); cerebroglycan; DKFZp547M109; FLJ38962; glypican proteoglycan 2; cerebroglycan proteoglycan
• Gene ID: 2818
• UniProt ID: Q8N158