Active Recombinant Mouse Glypican 2 (cerebroglycan), His-tagged

• Cat.No.: Gpc2-5625M
• Product Overview: RecombinantMouse Gpc2 protein was expressed in Murine myeloma cell line. Lys27-Arg358& Ala359-Leu554, with a C-terminal 6-His tag.

Specification

• Cat. No.: Gpc2-5625M
• Description: Theglypicans (GPC) constitute a family of heparan sulfate proteoglycans that areattached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor.Six members of this family have been identified in mammals (GPC1-GPC6). Allglypican core proteins contain an N-terminal signal peptide, a large globularcysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-likeregion containing the heparan sulfate attachment sites, and a C-terminal GPIattachment site. Based on the degree of their amino acid sequence similarity,two subfamilies of glypicans have been defined.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 um filtered solution in Tris-Citrate and NaCl.
• N-terminalSequence: Lys27 & Ala359
• MolecularWeight: 58.9 kDa
• Activity: Measuredby its binding ability in a functional ELISA. Immobilized rm Gpc2 at 4μg/mL(100μL/well) can bind rhMidkine with a linear range of 4-200 ng/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 60 kDa, non-reducingconditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100μg/mL in PBS.
• Storage: Use a manual defrost freezer and avoid repeated freezethawcycles. 12months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °Cunder sterile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution.
• Protein length: 27-358;359-554 a.a.

Gene Information

• Gene Name: Gpc2 glypican 2 (cerebroglycan)[ Mus musculus ]
• Official Symbol: Gpc2
• Synonyms: Gpc2; glypican 2(cerebroglycan); 2410016G05Rik; glypican-2
• Gene ID: 71951
• mRNA Refseq: NM_172412
• Protein Refseq: NP_766000
• UniProt ID: Q3TU56
• Chromosome Location: 5
• Function: heparan sulfateproteoglycan binding