Recombinant Human GOT1, His-tagged

• Cat.No.: GOT1-2577H
• Product Overview: Recombinant human GOT1, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW = 48.4kDa (433aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Glutamic-oxaloacetic transaminase (GOT) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQ
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: GOT1 glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) [ Homo sapiens ]
• Synonyms: GOT1; glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1); GIG18; aspartate aminotransferase, cytoplasmic; transaminase A; growth-inhibiting protein 18; glutamate oxaloacetate transaminase 1; EC 2.6.1.1
• Gene ID: 2805
• mRNA Refseq: NM_002079
• Protein Refseq: NP_002070
• MIM: 138180
• UniProt ID: P17174
• Chromosome Location: 10q24.1-q25.1
• Pathway: Alanine, aspartate and glutamate metabolism; Arginine and proline metabolism; Cysteine and methionine metabolism; Metabolic pathways; Phenylalanine metabolism; Phenylalanine, tyrosine and tryptophan biosynthesis; Tyrosine metabolism
• Function: L-aspartate:2-oxoglutarate aminotransferase activity; carboxylic acid binding; phosphatidylserine decarboxylase activity; pyridoxal phosphate binding