Recombinant Human GIYD2 293 Cell Lysate

Description :	Antigen standard for GIY-YIG domain containing 2 (GIYD2), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source :	HEK 293 cells
Species :	Human
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag :	Non

Gene Name	SLX1B SLX1 structure-specific endonuclease subunit homolog B (S. cerevisiae) [ Homo sapiens ]
Official Symbol	SLX1B
Synonyms	GIYD2; structure-specific endonuclease subunit SLX1; GIY-YIG domain-containing protein 1
Gene ID	79008
mRNA Refseq	NM_024044
Protein Refseq	NP_076949
MIM	615823
UniProt ID	Q9BQ83
Chromosome Location	16p11.2
Pathway	Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem