Recombinant Human EPH Receptor A1, GST-His

• Cat.No.: EPHA1-743H
• Product Overview: Recombinant Human EPHA1, Amino acids R569-D976, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells. MW = 79,631 Da.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 569-976 a.a.
• Description: EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis.
• Purification: One-step affinity purification using GSH-agarose.
• Product Identity: EPHA1 was confirmed as human EPHA1 by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
• Storage Buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.
• Concentration: 0.071 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 4 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: EPHA1 EPH receptor A1 [ Homo sapiens ]
• Synonyms: EPHA1; EPH receptor A1; EPH; EPHT; EPHT1; MGC163163; EphA1; EC 2.7.10.1; Tyrosine-protein kinase receptor EPH; eph tyrosine kinase 1; ephrin receptor EphA1; ephrin type-A receptor 1; erythropoietin-producing hepatoma amplified sequence; oncogene EPH; soluble EPHA1 variant 1; soluble EPHA1 variant 2
• Gene ID: 2041
• mRNA Refseq: NM_005232
• Protein Refseq: NP_005223
• MIM: 179610
• UniProt ID: P21709
• Chromosome Location: 7q32-q36
• Pathway: Axon guidance
• Function: ephrin receptor activity; nucleotide binding; protein binding; receptor activity; transferase activity