Active Recombinant Human EPHA1 Protein, GST-tagged

• Cat.No.: EPHA1-3374H
• Product Overview: Human EPHA1 (NP_005223.3, 586 a.a. - 976 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 586-976 a.a.
• Description: This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008]
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
  Substrate: Blk/Lyntide.
  ATP: 100 μM.
• Molecular Mass: 72 kDa
• Purity: 82 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: EPHA1 EPH receptor A1 [ Homo sapiens ]
• Official Symbol: EPHA1
• Synonyms: EPHA1; EPH receptor A1; EphA1 , EPHT, EPHT1; ephrin type-A receptor 1; EPH; hEpha1; oncogene EPH; eph tyrosine kinase 1; soluble EPHA1 variant 1; soluble EPHA1 variant 2; tyrosine-protein kinase receptor EPH; erythropoietin-producing hepatoma receptor; erythropoietin-producing hepatoma amplified sequence; EPHT; EPHT1; MGC163163
• Gene ID: 2041
• mRNA Refseq: NM_005232
• Protein Refseq: NP_005223
• MIM: 179610
• UniProt ID: P21709