Recombinant Human EIF2AK4 293 Cell Lysate

Description :	Antigen standard for eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source :	HEK 293 cells
Species :	Human
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag :	Non

Gene Name	EIF2AK4 eukaryotic translation initiation factor 2 alpha kinase 4 [ Homo sapiens ]
Official Symbol	EIF2AK4
Synonyms	EIF2AK4; eukaryotic translation initiation factor 2 alpha kinase 4; eukaryotic translation initiation factor 2-alpha kinase 4; GCN2; KIAA1338; GCN2-like protein; GCN2 eIF2alpha kinase;
Gene ID	440275
mRNA Refseq	NM_001013703
Protein Refseq	NP_001013725
MIM	609280
UniProt ID	Q9P2K8
Chromosome Location	15q13.3
Pathway	Hepatitis C, organism-specific biosystem; Hepatitis C, conserved biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; Influenza A, organism-specific biosystem; Influenza A, conserved biosystem; Measles, organism-specific biosystem;
Function	ATP binding; eukaryotic translation initiation factor 2alpha kinase activity; nucleotide binding; protein homodimerization activity; protein serine/threonine kinase activity; tRNA binding;

COA