Recombinant Human DOT1 like histone lysine methyltransferase Protein, His&StrepII tagged

• Cat.No.: DOT1L-120H
• Product Overview: Recombinant human DOT1L protein (Q8TEK3) (Met 1-Ser 351) with a Strep II tag at the N-terminus and a His tag at the C-terminus was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His&Strep II
• Protein Length: 1-351aa
• Description: DOT1L (mammalian counterpart of yeast Dot1) is a 1537 amino acid (aa), 165 kDa (predicted) nuclear non-SET domain histone H3K79 methyltransferase. It is involved in developmental gene transcription and maintenance of chromatin structure, and may promote mixed lineage leukemia. Within the sequence used as an immunogen, human DOT1L shares 97% and 94% aa identity with mouse and rat DOT1L, respectively. Two human isoforms affect this region: a 1467 aa form has an alternate start site at aa 71, while a 1513 aa form lacks aa 89-112. A third isoform of 1739 aa has a 202 aa C-terminal extension.
• Tag: N-StrepII and C-His
• Molecular Mass: 43.7 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: DOT1L DOT1 like histone lysine methyltransferase [ Homo sapiens (human) ]
• Official Symbol: DOT1L
• Synonyms: DOT1L; DOT1-like, histone H3 methyltransferase (S. cerevisiae); histone-lysine N-methyltransferase, H3 lysine-79 specific; DOT1; histone methyltransferase DOT1L; KIAA1814; KMT4; H3-K79-HMTase; DOT1-like protein; lysine N-methyltransferase 4; histone H3-K79 methyltransferase; DKFZp586P1823
• Gene ID: 84444
• mRNA Refseq: NM_032482
• Protein Refseq: NP_115871
• MIM: 607375
• UniProt ID: Q8TEK3