Recombinant Human DKK1, His-tagged, Biotinylated

• Cat.No.: DKK1-277H
• Product Overview: Human DKK1 /DKK-1 Protein, Biotinylated (Biotinylated DKK1) Thr 32 - His 266, was labeled with biotin based on rhDKK1 Protein, which was produced in human 293 cells (HEK293).

Specification

• Description: This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.?
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
• Molecular Mass: Biotinylated DKK1, fused with 6×His tag at the C-terminus, has a calculated MW of 25.8 kDa. The predicted N-terminus is Thr 32 or Ser 35. DTT-reduced Protein migrates as 40 kDa due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the Biotinylated DKK1 by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
• Protein length: 32-266 a.a.

Gene Information

• Gene Name: DKK1 dickkopf 1 homolog (Xenopus laevis) [ Homo sapiens ]
• Official Symbol: DKK1
• Synonyms: DKK1; dickkopf 1 homolog (Xenopus laevis); dickkopf (Xenopus laevis) homolog 1; dickkopf-related protein 1; DKK 1; SK; hDkk-1; dickkopf-1 like; dickkopf related protein-1; DKK-1
• Gene ID: 22943
• mRNA Refseq: NM_012242
• Protein Refseq: NP_036374
• MIM: 605189
• UniProt ID: O94907
• Chromosome Location: 10q11.2
• Pathway: Direct p53 effectors, organism-specific biosystem; Presenilin action in Notch and Wnt signaling, organism-specific biosystem; Regulation of Wnt-mediated beta catenin signaling and target gene transcription, organism-specific biosystem; Validated targets of C-MYC transcriptional repression, organism-specific biosystem; Wnt Signaling Pathway NetPath, organism-specific biosystem; Wnt signaling network, organism-specific biosystem; Wnt signaling pathway, organism-specific biosystem
• Function: growth factor activity; low-density lipoprotein particle receptor binding; protein binding; receptor antagonist activity; signal transducer activity