Recombinant Human Dickkopf Homolog 1 (Xenopus laevis), His-tagged

• Cat.No.: DKK1-347H
• Product Overview: Recombinant human Dkk-1 protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography (253 aa, 27.8 kDa).

Specification

• Species: Human
• Source: Hi-5 Insect Cells
• Tag: His
• Description: Dickkopf-related protein 1, also known as Dkk-1, is a member of the Dkk protein family including Dkk-1, 2, 3 and -4. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated level of Dkk-1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
• Formulation: Liquid. In PBS (pH 7.4) containing 10% glycerol.
• Molecular Weight: 27.8 kDa (253 aa).
• Purity: > 85% by SDS – PAGE.
• Concentration: 0.25 mg/ml (determined by Bradford assay).
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method).
• Sequence: ADPTLNSVLN SNAIKNLPPP LGGAAGHPGS AVSAAPGILY PGGNKYQTID NYQPYPCAED EECGTDEYCA SPTRGGDAGV QICLACRKRR KRCMRHAMCC PGNYCKNGIC VSSDQNHFRG EIEETITESF GNDHSTLDGY SRRTTLSSKM YHTKGQEGSV CLRSSDCASG LCCARHFWSK ICKPVLKEGQ VCTKHRRKGS HGLEIFQRCY CGEGLSCRIQ KDHHQASNSS RLHTCQRHSG RLVPRGSHHH HHH
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: DKK1
• Synonyms: DKK1; dickkopf homolog 1 (Xenopus laevis); SK; DKK-1; Dickkopf-1; Dkk-1; hDkk-1; OTTHUMP00000019617; dickkopf homolog 1; dickkopf (Xenopus laevis) homolog 1; dickkopf-1 like; dickkopf related protein-1; DKK1 dickkopf homolog 1 (Xenopus laevis).
• Gene ID: 22943
• mRNA Refseq: NM_012242.2
• Protein Refseq: NP_036374.1
• MIM: 605189
• UniProt ID: O94907
• Chromosome Location: 10q11.2
• Pathway: Wnt signaling pathway.
• Function: growth factor activity; low-density lipoprotein receptor binding; protein binding; signal transducer activity.