Active Recombinant Human DKK1 Protein, Biotinylated

• Cat.No.: DKK1-164H
• Product Overview: Recombinant Human DKK1(Thr32-His266) was expressed in Insect cells, Biotinylated.

Specification

• Description: Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains (CRDs) separated by a linker region. Each domain contains ten cysteine residues. Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 83% amino acid sequence identity with mouse and rat Dkk-1. Dkk-1 is a well-characterized antagonist of Wnt/beta-Catenin signaling. This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP-5 or LRP-6. Crystallographic studies have shown that Dkk-1 interacts with LRP-6 as a bipartite inhibitor, with both CRDs binding the extracellular domain of LRP-6 simultaneously. Dkk-1/LRP-6/Krm2 complex internalization has been shown to down-regulate Wnt signaling. Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development. Other sites of expression include developing neurons, hair follicles, and the retina. The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis. Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively. In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death. Dkk-1 also likely has a complex role in cancer, as it has been shown to act as a tumor suppressor and also to promote metastasis.
• Source: Insect cells
• Species: Human
• Predicted N Terminal: Thr32
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: easured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.2-1 μg/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt-3a.
• Molecular Mass: Predicted Molecular Mass: 26 kDa (unlabeled);
  SDS-PAGE: 33-38 kDa, reducing conditions.
• Protein length: Thr32-His266
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining
• Notes: Structure / Form: Monomer, biotinylated via sugars
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile
• Reconstitution: Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
• Tag: Non

Gene Information

• Gene Name: DKK1 dickkopf 1 homolog (Xenopus laevis) [ Homo sapiens ]
• Official Symbol: DKK1
• Synonyms: DKK1; dickkopf 1 homolog (Xenopus laevis); dickkopf (Xenopus laevis) homolog 1; dickkopf-related protein 1; DKK 1; SK; hDkk-1; dickkopf-1 like; dickkopf related protein-1; DKK-1
• Gene ID: 22943
• mRNA Refseq: NM_012242
• Protein Refseq: NP_036374
• MIM: 605189
• UniProt ID: O94907